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Test ID AATRP Alpha-1-Antitrypsin Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Useful For

Identification of abnormal accumulation of alpha-1-antitrypsin

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Alpha 1-Antitrypsin IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

Alpha-1-antitrypsin (AAT) is a plasma protein synthesized in the liver and is present in serum and tissue fluids where it acts as an inhibitor of proteases, especially elastase. AAT deficiency is associated with development of emphysema and liver disease. In liver disease, abnormal accumulation of AAT is seen as cytoplasmic globules in hepatocytes.

Interpretation

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Callea F, Fevery J, Massi G, Lievens C, de Groote J, Desmet VJ: Alpha-1-antitrypsin (AAT) and its stimulation in the liver of PiMZ phenotype individuals. A "recruitment-secretory block" ("R-SB") phenomenon. Liver. 1984:4(5):325-337

2. Lam M, Torbenson M, Yeh MM, Vivekanandan P, Ferrell L: HFE mutations in alpha-1-antitrypsin deficiency: an examination of cirrhotic explants. Mod Pathol. 2010;23:637-643

3. Theaker JM, Fleming KA: Alpha-1-antitrypsin and the liver: a routine immunohistological screen. J Clin Pathol. 1986;39(1):58-62

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AATRP Alpha 1-Antitrypsin IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
70664 Alpha 1-Antitripsin IHC, Tech Only Bill only; no result

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc