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Test ID ACTHI Adrenocorticotropic Hormone (ACTH) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Aids in the classification of pituitary adenomas and neoplasms with ectopic hormone production

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

ACTH IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Adrenocorticotropic hormone (ACTH) is a hormone produced and secreted by corticotrophs in the adenohypophysis (anterior lobe) of the pituitary gland. Normal pituitary exhibits positive staining in a large population of cells (approximately 15% to 20%). Immunohistochemical detection of ACTH may be useful in the classification of pituitary adenomas.


The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Hamid Z, Mrak RE, Ijaz MT, Faas FH: Sensitivity and Specificity of Immunohistochemistry in Pituitary Adenomas. The Endocrinologist 2009;19(1):38-43

2. Osamura RY, Kajiva H, Takei M, et al: Pathology of the human pituitary adenomas. Histochem Cell Biol 2008;130(3):495-507

3. Scheithauer BW, Jaap AJ, Horvath E, et al: Clinically Silent Corticotroph Tumors of the Pituitary Gland. Neurosurgery 2000;47(3):723-730

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ACTHI ACTH IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70665 ACTH IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: