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Test ID AFSH Alpha Follicle Stimulating Hormone Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Useful For

May aid in the classification of pituitary adenomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Method Name

Immunohistochemistry (IHC)

Reporting Name

Alpha FSH IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Follicle stimulating hormone (FSH) alpha subunit is a component common to all glycoprotein hormones produced by the anterior pituitary (luteinizing hormone [LH], thyroid-stimulating hormone [TSH], and FSH). Glycoprotein hormone-producing cells (approximately 30% of the total cell population) in normal pituitary stain in a cytoplasmic pattern. Immunohistochemical detection of alpha-FSH may be useful in the classification of pituitary adenomas.


This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Hamid Z, Mrak RE, Ijaz MT, Faas FH: Sensitivity and specificity of immunohistochemistry in pituitary adenomas. The Endocrinologist. 2009;19(1):38-43

2. Osamura RY, Kajiya H, Takei M, et al: Pathology of the human pituitary adenomas. Histochem Cell Biol. 2008;130(3):495-507

3. Osamura RY, Watanabe K: Immunohistochemical studies of human FSH producing pituitary adenomas. Virchows Archiv A. 1988;413(1):61-68

4. Pawlikowski M, Pisarek H, Kubiak R, Jaranowska M, Stepien H: Immunohistochemical detection of FSH receptors in pituitary adenomas and adrenal tumors. Folia Histochem Cytobiol. 2012;50(3):325-330

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
AFSH Alpha FSH IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
71849 Alpha FSH IHC, Tech Only Bill only; no result
Mayo Clinic Laboratories | IHC Catalog Additional Information: