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Test ID ALKLC Anaplastic Lymphoma Kinase for Lung Cancer, Immunohistochemistry

Shipping Instructions

Attach the green pathology address label included in the kit to the outside of the transport container.

Necessary Information

A pathology/diagnostic report and a brief history, including primary site of neoplasm, are required.

Specimen Required

Supplies: Pathology Packaging Kit (T554)

Specimen Type: Formalin-fixed, paraffin-embedded tissue block; or 3 unstained glass, “positively charged" slides with 4-microns, formalin-fixed, paraffin-embedded tissue

Additional Information: One slide will be stained with hematoxylin and eosin and returned.

Useful For

Identification of anaplastic lymphoma kinase overexpression


Diagnosis of inflammatory myofibroblastic tumor, anaplastic large-cell lymphoma, and for targeted therapy of lung adenocarcinoma

Method Name

Immunohistochemistry (IHC)

Reporting Name

ALK for Lung Cancer IHC

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
Special Ambient (preferred)

Clinical Information

A subset of anaplastic large-cell lymphomas overexpresses anaplastic lymphoma kinase (ALK-1) protein, resulting from a translocation involving the ALK1 gene. The abnormal ALK-1 expression can be in a nuclear or cytoplasmic distribution. Overexpression of ALK-1 protein is also useful for targeted therapy in lung adenocarcinoma and in the diagnosis of inflammatory myofibroblastic tumor. In normal tissue ALK-1 is negative.


This test, when not accompanied by a pathology consultation request, will be answered as either positive or negative. If additional interpretation or analysis is needed, request PATHC / Pathology Consultation along with this test.

Clinical Reference

1. Hutarew G, Hauser-Kronberger C, Strasser F, Llenos IC, Dietze O: Immunohistochemistry as a screening tool for ALK rearrangement in NSCLC: evaluation of five different ALK antibody clones and ALK FISH. Histopathology. 2014 Sept;65(3):398-407

2. Stein H, Foss H, Durkop H, et al: CD30(+) anaplastic large cell lymphoma: a review of its histopathologic, genetic, and clinical features. Blood. 2000 Dec;96(12):3681-3695

3. Yi ES, Boland JM, Maleszewski JJ, et al: Correlation of IHC and FISH for ALK gene rearrangement in non-small cell lung carcinoma: IHC score algorithm for FISH. J Thorac Oncol. 2011 Mar;6(3):459-465

Day(s) Performed

Monday through Friday

Report Available

5 to 7 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information


88341-If additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ALKLC ALK for Lung Cancer IHC 47303-3


Result ID Test Result Name Result LOINC Value
615714 Interpretation 59465-5
615715 Participated in the Interpretation No LOINC Needed
615716 Report electronically signed by 19139-5
615717 Material Received 81178-6
615718 Disclaimer 62364-5
615719 Case Number 80398-1

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHPCA IHC Additional No No
IHPCI IHC Initial No No

Testing Algorithm

For the initial immunohistochemistry (IHC) stain performed, the appropriate bill-only test ID will be added and charged (IHPCI). For each additional IHC stain performed, an additional bill-only test ID will be added and charged (IHPCA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: