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Test ID APPI Amyloid Precursor Protein (APP) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Aids in the identification of amyloid precursor protein present in Alzheimer disease

Method Name


Reporting Name

Amyloid Prec Protein IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Amyloid precursor protein (APP) is present in Alzheimer disease-associated plaques, large pyramidal cells as well as smaller neurons, astrocytes, and microglia. Histologic features of Alzheimer disease include the presence of abundant neurofibrillary, tangles, neuropil threads, and neuritic ("senile") plaques. The main component of senile plaque amyloid is a 39- to 42-amino acid segment referred to as beta amyloid, which is derived from APP.


The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Ahlgren S, Li GL, Olsson Y: Accumulation of beta-amyloid precursor protein and ubiquitin in axons after spinal cord trauma in humans: immunohistochemical observations on autopsy material. Acta Neuropathol 1996;92(1):49-55

2. Craggs LJ, Yamamoto Y, Ihara M, et al: White matter pathology and disconnection in the frontal lobe in cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL). Neuropathol Appl Neurobiol 2014;40:591-602

3. Dermaut B, Kumar-Singh S, De Jonghe C, et al: Cerebral amyloid angiopathy is a pathogenic lesion in Alzheimer's disease due to a novel presenilin 1 mutation. Brain 2001;124:2383-2392

4. Reichard RR, White CL 3rd, Hogan RN, et al: Beta-amyloid precursor protein immunohistochemistry in the evaluation of pediatric traumatic optic nerve injury. Ophthalmology 2004;111(4):822-827

5. Sawaguchi T, Franco P, Kadhim H, et al: Investigation into the correlation in SIDS victims between Alzheimer precursor protein A4 in the brainstem and sleep apnea. 2004;10:161-166

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
APPI Amyloid Prec Protein IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70670 Amyloid Prec Protein IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: