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Test ID BCATN Beta-Catenin Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Useful For

Identification of aberrant nuclear staining pattern observed in some tumors

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

B-Catenin IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Beta-catenin is involved in cellular cohesion through binding to the cytoplasmic tail of E-cadherin, and in intracellular signaling as a component of the Wnt pathway. In normal cells, B-catenin levels are regulated by the adenomatous polyposis coli (APC) protein, which promotes normal degradation of the protein. Alterations in either B-catenin or APC can result in accumulation of the protein and abnormal localization in the nucleus. In the nucleus, B-catenin acts as a cofactor in the upregulation of oncogenes including cyclin D1 and cmyc. In normal tissues, staining for B-catenin is limited to the membrane. Aberrant nuclear staining can be used diagnostically in selected tumors of the soft tissue (fibromatoses, endometrial stromal sarcoma), pancreas, liver and lung.


This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Klymkowsky MW: Beta-catenin and its regulatory network. Hum Pathol. 2005 Mar;36(3):225-227

2. Bell DA: Origins and molecular pathology of ovarian cancer. Mod Pathol. 2005 Feb;18(Suppl 2):S19-32

3. Smith ME, Pignatelli M: The molecular histology of neoplasia: the role of the cadherin/catenin complex. Histopathology. 1997 Aug;31(2):107-111

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BCATN B-Catenin IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70674 B-Catenin IHC, Tech Only Bill only; no result

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: