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Test ID BRAFV BRAF V600E Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Identification of BRAF V600E-mutated protein

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOA IHC Additional, Tech Only No, (Bill Only) No
IHTOI IHC Initial, Tech Only No, (Bill Only) No

Method Name


Reporting Name

BRAF V600E IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

BRAF is a serine/threonine protein kinase and a member of the Raf family. The BRAF V600E alteration leads to constitutive activation of the mitogen activated protein kinase pathway, which plays a role in cell proliferation and tumorigenesis. This genetic alteration has been detected in a variety of tumors such as melanoma, colorectal cancer, papillary thyroid carcinoma, hairy cell leukemia, Langerhans cell histiocytosis, and pleomorphic xanthoastrocytomas.


This test does not include pathologist interpretation: only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Ida C, Vrana JA, Rodriguez FJ, et al: Immunohistochemistry is highly sensitive and specific for detection of BRAF V600E mutation in pleomorphic xanthoastrocytoma. Acta Neuopath communications 2013;1:20

2. Capper D, Berghoff AS, Magerle M, et al: Immunohistochemical testing of BRAF V600E status in 1,120 tumor tissue samples of patients with brain metastases. Acta Neuropathol 2012;123(2):223-233

3. Andrulis M, Penzel R, Weichert W, et al: Application of a BRAF V600E Mutation-specific Antibody for the Diagnosis of Hairy Cell Leukemia. Am J Surg Pathol 2012;36(12):1796-1800

4. Koperek O, Kornauth C, Capper D, et al: Immunohistochemical detection of the BRAF V600E-mutated protein in papillary thyroid carcinoma. Am J Surg Pathol 2012;36(6):844-850

5. Skorokhod A, Capper D, von Deimling A, et al: Detection of BRAF V600E mutations in skin metastases of malignant melanoma by monoclonal antibody VE1. J Am Acad skin metastases of malignant melanoma by monoclonal antibody VE1. J Am Acad Dermatol 2012;67(3):488-491

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BRAFV BRAF V600E IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70680 BRAF V600E IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Disease States

  • Colorectal cancer

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: