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Test ID BTIA1 T-Cell Intracellular Antigen 1 (TIA-1) Immunostain, Bone Marrow, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Characterizing neoplasms of cytotoxic T cells or natural killer cells

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

Bone Marrow TIA1 IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

T-cell intracellular antigen 1 (TIA-1) shows a granular cytoplasmic staining pattern due to its presence in cytotoxic granules. It is involved in cytotoxic cell-mediated immune responses. TIA-1 was first identified in cytotoxic T cells; it is also expressed in normal natural killer (NK) cells and granulocytes.


This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to test code PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Fujimura T, Furudate S, Kambayashi Y, Aiba S: Potential use of bisphosphonates in invasive extramammary Paget's disease: An immunohistochemical investigation. Clin Dev Immunol. 2013;2013(3):164982. doi: 10.1155/2013/164982

2. Kiyasu J, Aoki R, Tanaka PY, et al: FOXP3+ regulatory and TIA-1+ cytotoxic T lymphocytes in HIV-associated Hodgkin lymphoma. Pathol Inter. 2012;62(1):77-83. doi: 10.1111/j.1440-1827.2011.02754.x

3. Zlobec I, Karamitopoulou E, Terracciano L, et al: TIA-1 cytotoxic granule-associated RNA binding protein improves the prognostic performance of CD8 in mismatch repair-proficient colorectal cancer. PLoS One. 2010;5(12):e14282. doi: 10.1371/journal.pone.0014282

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BTIA1 Bone Marrow TIA1 IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
605161 Bone Marrow TIA1 IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Mayo Clinic Laboratories | IHC Catalog Additional Information: