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Test ID CALCI Calcitonin (CALCI) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Aids in the identification of C cells of thyroid, medullary thyroid carcinomas, many atypical laryngeal carcinoids, and other neuroendocrine tumors

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

Calcitonin IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Calcitonin is a hormone involved in calcium metabolism. Staining for calcitonin produces fine granular, cytoplasmic staining of C cells of thyroid, medullary thyroid carcinomas, many atypical laryngeal carcinoids, and other neuroendocrine tumors. Amyloid deposits within medullary thyroid carcinoma may also exhibit varying degrees of calcitonin immunoreactivity.


This test does not include pathologist interpretation; only technical performance of the stain is performed. If an interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Costante G, Meringolo D: Calcitonin as a biomarker of C cell disease: recent achievements and current challenges. Endocrine. 2020;(67):273-280. doi: 10.1007/s12020-019-02183-6

2. Gambardella C, Offi C, Patrone R, et al: Calcitonin negative medullary thyroid carcinoma: a challenging diagnosis or a medical dilemma?. BMC Endocr Disord. 2019;(19):45. doi: 10.1186/s12902-019-0367-2

3. Gambardella C, Offi C, Clarizia G, et al: Medullary thyroid carcinoma with double negative calcitonin and CEA: a case report and update of literature review. BMC Endocr Disord. 2019;(19):103. doi: 10.1186/s12902-019-0435-7

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CALCI Calcitonin IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70681 Calcitonin IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.
Mayo Clinic Laboratories | IHC Catalog Additional Information: