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Test ID CALD Caldesmon Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Useful For

A marker of smooth muscle differentiation

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry

Reporting Name

Caldesmon IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

Caldesmon is a smooth muscle specific protein that regulates smooth muscle contraction. This clone recognizes the high-molecular-weight variant (h-caldesmon) and does not react with the nonmuscle variant. Neither variant of caldesmon is present in skeletal muscle. Anti-h-caldesmon seems to be a reliable marker of smooth muscle differentiation, and may assist in the diagnosis of smooth muscle tumors.

Interpretation

This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Wick MR, Hornick JL: Immunohistology of soft tissue and osseous neoplasms. In: Dabbs DJ, ed. Diagnostic Immunohistochemistry: Theranostic and Genomic Applications. 3rd ed. 2010:chap 4 2. Horita A, Kurata A, Maeda D,Fukayama M, Sakamoto A: Immunohistochemical characteristics of atypical polypoid adenomyoma with special reference to h-caldesmon. Int J Gynecol Pathol 2011 Jan;30(1)64-70

3. Ordonez NG: Value of PAX8, PAX2, claudin-4, and h-caldesmon immunostaining in distinguishing peritoneal epithelioid mesotheliomas from serous carcinomas. Modern Pathology. 2013;26:553-562

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CALD Caldesmon IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
70682 Caldesmon IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc