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HelpTest ID CD99 CD99 (MIC-2) Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Specimen Type: Tissue
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Preferred:
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: None
Useful For
Identification of Ewing sarcoma and primitive neuroectodermal tumors
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Method Name
Immunohistochemistry (IHC)
Reporting Name
CD99 (MIC-2) IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
CD99 (cluster of differentiation 99) is the product of the MIC2 gene. It is expressed in normal tissues including some lymphocytes, pancreatic islet cells, granulosa cells of the ovary, Sertoli cells of the testis, and ependymal cells of the central nervous system. It is strongly expressed in Ewing sarcoma/primitive neuroectodermal tumor, distinguishing it from other small round blue cell tumors of childhood and adolescence.
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clinical Reference
1. Nunez J, Veloda C, Liu Y, Wakely PE Jr, Silverman JF. Utility of an immunohistochemical panel to distinguish nonductal pancreatic neoplasms in surgical pathology. Am J Clin Pathol. 2013;140:A181. doi:10.1093/ajcp/140.suppl1.181
2. Karikari IO, Mehta AI, Nimjee S, et al. Primary intradural extraosseous Ewing sarcoma of the spine: Case report and literature review. Neurosurgery. 2011;69(4):E995-999. doi:10.1227/NEU.0b013e318223b7c7
3. Romero-Rojas AE, Diaz-Perez JA, Ariza-Serrano LM. CD99 is expressed in chordoid glioma and suggests ependymal origin. Virchows Arch. 2012;460(1):119-122. doi:10.1007/s00428-011-1170-2
4. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, primary
88341-TC, if additional IHC
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| CD99 | CD99 (MIC-2) IHC, Tech Only | Order only;no result |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 70820 | CD99 (MIC-2) IHC, Tech Only | Bill only; no result |
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
mcl-ihc