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Test ID DNJB9 DNAJB9 Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Useful For

Diagnosis of fibrillary glomerulonephritis

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

DNAJB9 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

DNAJB9 (Dnaj [hsp40] homolog, subfamily b, member 9) is a heat shock protein that plays a role in protein folding. Proteomic analysis indicates that the DNAJB9 protein is specifically identified in glomeruli of patients with fibrillary glomerulonephritis (FGN). By immunohistochemistry there is an intense smudgy staining of extracellular glomerular deposits for DNAJB9 in patients with FGN.

Interpretation

This test does not include pathologist interpretation; only technical performance of the stain is performed. If an interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Nasr SH, Vrana JA, Dasari S, et al: DNAJB9 is a specific immunohistochemical marker for fibrillary glomerulonephritis. Kidney Int Rep. 2018 Jan;3(1):56-64

2. Dasari S, Alexander MP, Vrana JA, et al: DnaJ heat shock protein family B member 9 is a novel biomarker for fibrillary GN. J Am Soc Nephrol. 2018 Jan;29(1):51-56

3. Liang S, Chen D, Liang D, et al: Clinicopathological characteristics and outcome of patients with fibrillary glomerulonephritis: DNAJB9 is a valuable histologic marker. J Nephrol. 2020 Jun 18. doi: 10.1007/s40620-020-00783-4

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
DNJB9 DNAJB9 IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
71740 DNAJB9 IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send 1 of the following forms with the specimen:

-Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763)

-Renal Diagnostics Test Request (T830)

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc