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Test ID EMAI Epithelial Membrane Antigen (EMA) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Recognizing epithelial derivation of poorly differentiated malignant tumors


Subtyping intraductal papillary mucinous neoplasms when used in conjunction with mucin (MUC) 2, MUC5AC and MUC6

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

EMA IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Epithelial membrane antigen (EMA), also known as mucin 1 (MUC1), is expressed by epithelial cells of all types, mesothelial cells, perineural cells, and a subset of plasma cells. EMA is expressed by meningiomas, synovial sarcoma, epithelioid sarcoma, a subset of peripheral nerve sheath tumors, the lymphocyte-predominant cells of lymphocyte-predominant Hodgkin lymphoma, and anaplastic large cell lymphoma. Diagnostically, EMA is useful in recognizing epithelial derivation of poorly differentiated malignant tumors and, in conjunction with a panel of mucin markers (MUC2, MUC5AC, and MUC6), may be used in subtyping intraductal papillary mucinous neoplasms.


This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, .call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Kwak H, Liu X, Allende D, et al:  Interobserver variability in intraductal papillary mucinous neoplasm subtypes and application of their mucin immunoprofiles. Modern Pathol. 2016; 29:977-984

2. Ramezani M, Mohamadzaheri E, Khazaei S, et al: Comparison of EMA,CEA, CD10 and Bcl-2 Biomarkers by immunohistochemistry in squamous cell carcinoma and basal cell carcinoma of the skin. Asian Pac J Cancer Prev. 2016;17(3):1379-83. doi: 10.7314/apjcp.2016.17.3.1379

3. Song W, Flucke U, Suurmeijer AJH. Myoepithelial tumors of bone. Surg Pathol Clin. 2017 Sep;10(3):657-674. doi: 10.1016/j.path.2017.04.010

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
EMAI EMA IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70737 EMA IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: