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HelpTest ID EMAI Epithelial Membrane Antigen (EMA) Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Preferred:
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: None
Useful For
Recognizing epithelial derivation of poorly differentiated malignant tumors
Subtyping intraductal papillary mucinous neoplasms when used in conjunction with mucin (MUC) 2, MUC5AC and MUC6
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Method Name
Immunohistochemistry (IHC)
Reporting Name
EMA IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
Epithelial membrane antigen (EMA), also known as mucin 1, is expressed by epithelial cells of all types, mesothelial cells, perineural cells, and a subset of plasma cells. EMA is expressed by meningiomas, synovial sarcoma, epithelioid sarcoma, a subset of peripheral nerve sheath tumors, the lymphocyte-predominant cells of lymphocyte-predominant Hodgkin lymphoma, and anaplastic large cell lymphoma. Diagnostically, EMA is useful in recognizing epithelial derivation of poorly differentiated malignant tumors and, in conjunction with a panel of mucin markers (MUC2, MUC5AC, and MUC6), may be used in subtyping intraductal papillary mucinous neoplasms.
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clinical Reference
1. Kwak HA, Liu X, Allende DS, Pai RK, Hart J, Xiao SY. Interobserver variability in intraductal papillary mucinous neoplasm subtypes and application of their mucin immunoprofiles. Mod Pathol. 2016;29(9):977-984. doi:10.1038/modpathol.2016.93
2. Ramezani M, Mohamadzaheri E, Khazaei S, et al. Comparison of EMA,CEA, CD10 and Bcl-2 Biomarkers by Immunohistochemistry in Squamous Cell Carcinoma and Basal Cell Carcinoma of the Skin. Asian Pac J Cancer Prev. 2016;17(3):1379-1383. doi:10.7314/apjcp.2016.17.3.1379
3. Song W, Flucke U, Suurmeijer AJH. Myoepithelial Tumors of Bone. Surg Pathol Clin. 2017;10(3):657-674. doi:10.1016/j.path.2017.04.010
4. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, primary
88341-TC, if additional IHC
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| EMAI | EMA IHC, Tech Only | Order only;no result |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 70737 | EMA IHC, Tech Only | Bill only; no result |
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Testing Algorithm
For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).
mcl-ihc