Home
HelpTest ID ERBE1 Estrogen Receptor Beta-1 Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Preferred:
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: None
Useful For
Detection of estrogen receptor-beta 1 protein levels in cancer, including triple-negative breast cancer
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Method Name
Immunohistochemistry (IHC)
Reporting Name
Estrogen Rec Beta1 IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
This test is intended to identify the presence of estrogen receptor beta 1 (ER-beta 1) protein. ER-beta 1 is a member of the nuclear receptor superfamily of transcription factors and is the product of the ESR2 gene on chromosome 14q22-24. Unlike ER-alpha, ER-beta 1 is highly expressed in normal breast epithelium but expression is reduced in many precancerous and cancerous breast tumors. ER-beta 1 is expressed in 30% to 40% of triple negative breast cancers and is associated with improved outcomes in ER-alpha positive tamoxifen treated patients.
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clinical Reference
1. Kim TJ, Lee A, Choi YJ, Song BJ, et al. Prognostic significance of high expression of ER-beta in surgically treated ER-positive breast cancer following endocrine therapy. J Breast Cancer. 2012;15(1):79-86. doi:10.4048/jbc.2012.15.1.79
2. Reese JM, Suman VJ, Subramaniam M, et al. ER beta1: Characterization, prognosis, and evaluation of treatment strategies in ER alpha-positive and-negative breast cancer. BMC Cancer. 2014;14:749-764
3. Wu X, Subramaniam M, Negron V, et al. Development, characterization, and applications of a novel estrogen receptor beta monoclonal antibody. J Cell Biochem. 2012;113:711-723
4. Marotti J, Collins L, Hu R. Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: Results from the Nurses' Health Study. Mod Pathol. 2010; 23,197-204
5. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of Immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, primary
88341-TC, if additional IHC
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| ERBE1 | Estrogen Rec Beta1 IHC, Tech Only | Order only;no result |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 71492 | Estrogen Rec Beta1 IHC, Tech Only | Bill only; no result |
Forms
If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Testing Algorithm
For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).
mcl-ihc