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Test ID ERBE1 Estrogen Receptor Beta-1 Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Detection of estrogen receptor-beta 1 protein levels in cancer, including triple-negative breast cancer

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Estrogen Rec Beta1 IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

This test is intended to identify the presence of estrogen receptor beta 1 (ER-beta 1) protein. ER-beta 1 is a member of the nuclear receptor superfamily of transcription factors and is the product of the ESR2 gene on chromosome 14q22-24. Unlike ER-alpha, ER-beta 1 is highly expressed in normal breast epithelium but expression is reduced in many precancerous and cancerous breast tumors. ER-beta 1 is expressed in 30% to 40% of triple negative breast cancers and is associated with improved outcomes in ER-alpha positive tamoxifen treated patients.


This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Kim TJ, Lee A, Choi YJ, Song BJ, et al: Prognostic significance of high expression of ER-beta in surgically treated ER-positive breast cancer following endocrine therapy. J Breast Cancer. 2012 Mar;15(1):79-86. doi: 10.4048/jbc.2012.15.1.79

2. Reese JM, Suman VJ, Subramaniam M, et al: ER beta1: Characterization, prognosis, and evaluation of treatment strategies in ER alpha-positive and-negative breast cancer. BMC Cancer. 2014;14:749-764

3. Wu X, Subramaniam M, Negron V, et al: Development, characterization, and applications of a novel estrogen receptor beta monoclonal antibody. J Cell Biochem. 2012;113:711-723

4. Marotti J, Collins L, Hu R: Estrogen receptor-beta expression in invasive breast cancer in relation to molecular phenotype: Results from the Nurses' Health Study. Mod Pathol. 2010; 23,197-204

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ERBE1 Estrogen Rec Beta1 IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
71492 Estrogen Rec Beta1 IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: