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Test ID HEPBS Hepatitis B Surface (HBs) Antigen Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Aiding in the identification of hepatitis B infection (carrier state)

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

Hepatitis B surface IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Hepatitis B surface antigen (HBsAg) stains the cytoplasm of hepatitis B-infected hepatocytes in a granular pattern. The complete infective virion consists of a core of double-stranded DNA, a specific DNA polymerase, and structural proteins. The nucleocapsid contains 2 serologically distinct antigens, the core antigen (HBcAg) and envelope antigen (HBeAG). These are surrounded by an outer envelope of surface protein that is recognized serologically as hepatitis B virus surface antigen. Core antigen is most often demonstrated in chronic active hepatitis, compared to surface antigen in the carrier state.


This test does not include pathologist interpretation; only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request, call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. He Z, Chen J, Wang J, et al: Expression of hepatitis B surface antigen in liver tissues can serve as a predictor of prognosis for hepatitis B virus-related hepatocellular carcinoma patients after liver resection. Eur J Gastroenterol Hepatol. 2021 Jan;33(1):76-82 doi: 10.1097/MEG.0000000000001698

2. Wang Q, Sachse P, Semmo M, et al: T- and B-cell responses and previous exposure to hepatitis B virus in 'anti-HBc alone' patients. J Viral Hepat. 2015 Dec;22(12):1068-1078. doi: 10.1111/jvh.12428

3. Kabaçam G, Wedemeyer H, Savas B, et al: Role of immunohistochemistry for hepatitis D and hepatitis B virus in hepatitis delta. Liver Int. 2014 Sep;34(8):1207-1215. doi: 10.1111/liv.12376

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HEPBS Hepatitis B surface IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70766 Hepatitis B surface IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: