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Test ID HMGA High Mobility Group A2 (HMGA2) Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Useful For

Identification of abnormal expression of the high mobility group protein, HMGA2

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

HMGA2 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

High mobility group (HMG) proteins are nonhistone chromatin factors expressed during embryonic development that function to regulate transcription and cellular differentiation. When expression of HMGA2 is deregulated in cells of adult tissues, this oncofetal protein promotes neoplastic transformation. Abnormal HMGA2 expression has been observed in a wide variety of neoplasms, including uterine leiomyomas, ovarian serous carcinomas, lipomatous tumors, lymphangiomyomatosis, and pancreatic carcinomas. Normal spermatids express HMGA2. This immunostain may be useful in the differential diagnosis of lower genital tract mesenchymal tumors, showing positivity in the majority of aggressive angiomyxomas.

Interpretation

This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Ding X, Wang Y, Ma X, et al: Expression of HMGA 2 in bladder cancer and its association with epithelial-to-mesenchymal transition. Cell Prolif. 2014; 7(2):146-151. doi: 10.1111/cpr.12096

2. Lu B, Shi H, Zhang X: Myxoid leiomyosarcoma of the uterus: a clinicopathological and immunohistochemical study of 10 cases. Human Pathol. 2017; 59:139-146. doi: 10.1016/j.humpath.2016.09.014

3. Gong J, Wang Y, Jiang B, et al: Impact of high-mobility-group A2 overexpression on epithelial-mesenchymal transition in pancreatic cancer. Cancer Manag Res. 2019;11:4075-4084. doi: 10.2147/CMAR.S199289

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HMGA HMGA2 IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
70773 HMGA2 IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc