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Test ID HRPSV Herpes Simplex Virus, I and II (HSV I and II) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Identification of herpes simplex virus I and II infection

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

HSV I and II IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Immunoperoxidase staining for herpes simplex virus (HSV) I and II produces nuclear and granular cytoplasmic staining of virus infected cells. HSV I and II are part of the herpes virus family that also includes Epstein Barr virus, herpes zoster, and cytomegalovirus. HSV are among the most common infectious agents of man and usually are transmitted through close personal contact. The manifestations of infection can be localized (oral lesions HSV I; genital lesions HSV II) or can cause life-threatening systemic infection in immunocompromised patients.


This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Molina-Ruiz AM, Santonja C, Rutten A, Cerroni L, Kutzner H, Requena L: Immunohistochemistry in the diagnosis of cutaneous viral infections-part I. Cutaneous viral infections by herpesviruses and papillomaviruses. Am J Dermatopathol. 2015 Jan 1;37(1):1-4. doi: 10.1097/DAD.0000000000000203

2. Lloyd J, Copaciu R, Yahyabeik A, et al: Characterization of polyclonal antibodies to herpes simplex virus types 1 and 2. J Histotechnol. 2019 Oct 2;42(4):202-214. doi: 10.1080/01478885.2019.1683132

3. Zhang DH, Zhang QY, Hong CQ, Chen JY, Shen ZY, Zhu Y: Prevalence and association of human papillomavirus 16, Epstein-Barr virus, herpes simplex virus-1 and cytomegalovirus infection with human esophageal carcinoma: a case-control study. Oncol Rep. 2011 Jun 1;25(6):1731-1738. doi: 10.3892/or.2011.1234

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
HRPSV HSV I and II IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70786 HSV I and II IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: