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Test ID ICOSI ICOS (CD278), Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostains Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostains Technical Only Envelope

Preferred: 2 unstained positively charged glass slide (25 x 75 x 1 mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Useful For

Classification of T-cell lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

ICOS (CD278) IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

ICOS (inducible T-cell costimulator or CD278: cluster of differentiation 278) is primarily expressed on activated CD4+ and CD8+ T cells where it regulates immune responses and plays a role in the regulation of T-follicular helper cells. ICOS is a sensitive marker for identifying T-cell lymphomas of follicular helper T-cell origin, especially certain patterns of angioimmunoblastic T-cell lymphoma (AITL) and peripheral T-cell lymphomas with T-follicular helper phenotype (PTCL-TFH).

Interpretation

This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Baseggio L, Traverse-Glehen A, Berger F, et al: CD10 and ICOS expression by multiparametric flow cytometry in angioimmunoblastic T-cell lymphoma. Modern Pathology. 2011 Jul;24(7):993-1003

2. Tan LH, Tan SY: Aberrant immunoarchitecture distinguishes hyperplastic germinal centres in pattern 1 angioimmunoblastic T-cell lymphoma from reactive follicles. Hematol Oncol. 2014;32(3):145-154

3. Bosisio FM, Cerroni L: Expression of T-follicular helper markers in sequential biopsies of progressive mycosis fungoides and other primary cutaneous T-cell lymphomas. Am J Dermatopathol. 2015;37(2):115-121

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ICOSI ICOS (CD278) IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
113596 ICOS (CD278) IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc