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HelpTest ID KLISH Kappa and Lambda Light Chain mRNA, In Situ Hybridization (ISH) Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green "Attention Pathology" address label (T498) and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Specimen Type: Tissue
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Preferred:
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: None
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
Useful For
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| ISTOI | ISH Initial, Tech Only | No | No |
| ISTOA | ISH Additional, Tech Only | No | No |
Method Name
In Situ Hybridization (ISH)
Reporting Name
Kappa/Lambda ISH, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
Interpretation
This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient’s clinical history and other diagnostic tests by a qualified pathologist.
Clinical Reference
1. Akhtar N, Ruprai A, Pringle JH, et al. In situ hybridization detection of light chain mRNA in routine bone marrow trephines from patients with suspected myeloma. Br J Haematol. 1989;73:296-301
2. Weiss LM, Movahed LA, Chen YY, et al. Detection of immunoglobulin light-chain mRNA in lymphoid tissues using a practical in-situ hybridization method. Am J Pathol. 1990;137:979-988
3. Hristov AC, Comfere NI, Vidal CI, Sundram U. Kappa and lambda immunohistochemistry and in situ hybridization in the evaluation of atypical cutaneous lymphoid infiltrates. J Cutan Pathol. 2020;47(11):1103-1110. doi:10.1111/cup.13858
4. Magaki S, Hojat SA, Wei B, So A, Yong WH. An introduction to the performance of immunohistochemistry. Methods Mol Biol. 2019;1897:289-298. doi:10.1007/978-1-4939-8935-5_25
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysTest Classification
This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
88365-TC, primary
88364-TC, if additional ISH
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| KLISH | Kappa/Lambda ISH, Tech Only | Order only;no result |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 70792 | Kappa/Lambda ISH, Tech Only | Bill only; no result |
Testing Algorithm
For the initial technical component only in situ hybridization (ISH) stain performed, the appropriate bill only test ID will be reflexed and charged (ISTOI). For each additional technical component only ISH stain performed, an additional bill only test ID will be reflexed and charged (ISTOA).
mcl-ish-without