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Test ID LMO2 LIM Domain Only 2 (LMO2) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

LMO2 IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

LIM domain only 2 (LMO2) is a transcription factor that regulates vascular and hematopoietic systems and is involved in hematolymphoid neoplasia. LMO2 is preferentially expressed by germinal center B cells and may also be expressed in erythroid and myeloid precursors and in megakaryocytes. Expression has been observed in cases of lymphoblastic and acute myeloid leukemia. It is rarely expressed in mature T, natural killer, and plasma cell neoplasms and is absent from nonhematolymphoid tissues except for endothelial cells. In the diagnosis of B-cell lymphomas, LMO2 can be useful in an immunohistochemical panel to assign a germinal center phenotype.


This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Natkunam Y, Zhao S, Mason DY, et al: The oncoprotein LMO2 is expressed in normal germinal-center b cells and in human b-cell lymphomas. Blood 2007, Feb 15;109(4):1636-1642

2. Blenk S, Engelmann J, Weniger M, et al: Germinal center b cell-like and activated b cell-like type of diffuse large b cell lymphoma: Analysis of molecular predictors, signatures, cell cycle state and patient survival. Cancer Inform 2007 Dec 12;3:399-420

3. Natkunam Y, Farinha P, His ED, et al: LMO2 protein expression predicts survival in patients with diffuse large b -cell lymphoma treated with anthracycline-based chemotherapy with and without rituximab. J Clin Oncol 2008 Jan 20;26(3):447-454

4. Gratzinger D, Zhao S, West R, et al: The transcription factor LMO2 is a robust marker of vascular endothelium and vascular neoplasms and selected other entities. Am J Clin Pathol 2009 Feb;131(2):264-278

5. Younes SF, Beck AH, Lossos IS, et al: Immunoarchitectural patterns in follicular lymphoma: efficacy of HGAL and LMO2 in the detection of the interfollicular and diffuse components. Am J Surg Pathol 2010 Sep;34(9):1266-1276

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LMO2 LMO2 IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70813 LMO2 IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).

Day(s) Performed

Monday through Friday

Mayo Clinic Laboratories | IHC Catalog Additional Information: