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Test ID OCT2 OCT-2 Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded tissue block


Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

OCT-2 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

OCT-2 is a transcription factor that binds to the octamer motif of the immunoglobulin gene promoter, recruits the coactivator BOB.1, and activates immunoglobulin gene transcription. OCT-2 is variably expressed in germinal center B cells, weakly expressed in mantle zone B cells, and weakly to moderately expressed in plasma cells. The protein is localized to the nuclear compartment. Expression of BOB.1, OCT-2, and PU.1 transcription factors are often down regulated in classical Hodgkin lymphoma. OCT-2 is overexpressed in lymphocyte-predominant (LP) cells of nodular LP Hodgkin lymphoma. These properties can be useful in the diagnosis of lymphoma.

Interpretation

This test does not include pathologist interpretation, only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.

 

The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. McCune RC, Syrbu SE, Vasef MA: Expression profiling of transcription factors Pax-5, Oct-1, Oct-2, BOB.1, and PU.1 in Hodgkin's and non-Hodgkin's lymphomas: a comparative study using high throughput tissue microarrays. Mod Pathol. 2006 Jul;19(7):1010-1018

2. Loddenkemper C, Anagnostopoulos I, Hummell M, et al: Differential Eu enhancer activity and expression of BOB.1/OBF.1, Oct2, PU.1, and immunoglobulin in reactive B-cell populations, B-cell non Hodgkin lymphomas and Hodgkin lymphomas. J Pathol. 2004 Jan;202(1):60-69

3. Marafioti T, Ascani S, PulfordK, et al: Expression of B-lymphocyte-associated transcription factors in human T-cell neoplasm. Am J Pathol. 2003 Mar;162(3):861-871

4. O'Malley DP, Fedoriw Y, Weiss LM: Distinguishing classical Hodgkin lymphoma, gray zone lymphoma, and large B-cell lymphoma: A proposed scoring system. Appl Immunohistochem Mol Morphol..2016 Sep;24(8):535-540. doi: 10.1097/PAI.0000000000000236

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OCT2 OCT-2 IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
70834 OCT-2 IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc