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HelpTest ID PRAME PRAME Immunostain, Technical Component Only
Ordering Guidance
This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.
Shipping Instructions
Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.
Specimen Required
Specimen Type: Tissue
Supplies: Immunostain Technical Only Envelope (T693)
Container/Tube: Immunostain Technical Only Envelope
Preferred:
-Formalin-fixed, paraffin-embedded tissue block
OR
-2 Unstained, positively charged glass slides (25- x 75- x 1-mm) per test ordered; sections 4-microns thick
Acceptable: None
Useful For
Differentiation between melanoma and benign nevi.
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| IHTOI | IHC Initial, Tech Only | No | No |
| IHTOA | IHC Additional, Tech Only | No | No |
Testing Algorithm
For the initial technical component only immunohistochemical (IHC) stain performed, the appropriate bill-only test ID will be reflexed and charged (IHTOI). For each additional technical component only IHC stain performed, an additional bill-only test ID will be reflexed and charged (IHTOA).
Method Name
Immunohistochemistry (IHC)
Reporting Name
PRAME IHC, Tech OnlySpecimen Type
TECHONLYSpecimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| TECHONLY | Ambient (preferred) | ||
| Refrigerated | |||
Clinical Information
PRAME (Preferentially expressed Antigen in Melanoma) expression in melanocytic neoplasms is strongly associated with melanoma and may be useful in differentiating benign nevi from malignant melanocytic lesions.
Interpretation
This test does not include pathologist interpretation only technical performance of the stain. If interpretation is required, order PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case.
The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.
Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.
Clinical Reference
1. Lezcano C, Jungbluth AA, Nehal KS, et al: PRAME expression in melanocytic tumors. Am J Surg Pathol. 2018 Nov;42(11):1456-1465. doi: 10.1097/PAS.0000000000001134
2. Raghavan SS, Wang JY, Toland A, et al: Diffuse PRAME expression is highly specific for malignant melanoma in the distinction from clear cell sarcoma. J of Cutan Pathol. 2020 Dec;47(12):1226-1228. doi: 10.1111/cup.13812
3. Gradecki SE, Slingluff CL Jr, Gru AA: PRAME expression in 155 cases of metastatic melanoma. J Cutan Pathol. 2021 Apr;48(4):479-485. doi: 10.1111/cup.13876
4. Lezcano C, Jungbluth AA, Busam KJ: Comparison of immunohistochemistry for PRAME with cytogenetic test results in the evaluation of challenging melanocytic tumors. Am J Surg Pathol. 2020 Jul;44(7):893-900. doi: 10.1097/PAS.0000000000001492
5. Lezcano C, Jungbluth AA, Busam KJ: PRAME immunohistochemistry as an ancillary test for the assessment of melanocytic lesions. Surg Pathol Clin. 2021 Jun;14(2):165-175. doi: 10.1016/j.path.2021.01.001
Day(s) Performed
Monday through Friday
Report Available
1 to 3 daysTest Classification
This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.CPT Code Information
88342-TC, primary
88341-TC, if additional IHC
LOINC Code Information
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| 615795 | PRAME IHC, Tech Only | No LOINC Needed |
Forms
If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.
mcl-ihc