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Test ID RSVAB Respiratory Syncytial Virus (RSV) In Situ Hybridization, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope

Preferred: 4 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Aids in the identification of respiratory syncytial viral infection

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ISTOI ISH Initial, Tech Only No No
ISTOA ISH Additional, Tech Only No No

Method Name

In Situ Hybridization

Reporting Name

Resp Syncytial Virus(RSV)ISH,T Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

Respiratory syncytial virus (RSV) causes a wheezing illness that especially affects young children. The virus infects alveolar pneumocytes and induces fusion of multiple cells, creating a "syncytial" multinucleated cell. This RSV probe sequence was designed to identify subgroups RSV-A and RSV-B. It has been verified to not cross react with the following viruses in formalin-fixed, paraffin-embedded (FFPE) tissue: adenovirus (ADV), BK virus (BKV), cytomegalovirus (CMV), Epstein-Barr virus (EBV), hepatitis C virus (HCV), human herpes virus, type 8 (HHV-8), human papillomavirus (HPV) types 6, 11, 16, E6/E7, Helicobacter pylori (H pylori), herpes simplex virus (HSV) types 1 and 2, JC virus (JCV), parvovirus, toxoplasma, and varicella-zoster viruses (VZV).


This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Hall CB: Respiratory syncytial virus and parainfluenza virus. N Engl J Med. 2001 Jun 21;344(25):1917-1928

2. Essaidi-Laziosi M, Lyon M, Mamin A, et al: Journal of Virological Methods. 2016;235;9-14

3. User manual: RNAscope 2.5 VS Assay for Ventana DISCOVER ULTRA System BROWN, Document Number 322200-USM_ULT

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

88365-TC, Primary

88364-TC, if additional ISH

LOINC Code Information

Test ID Test Order Name Order LOINC Value
RSVAB Resp Syncytial Virus(RSV)ISH,T Only Order only;no result


Result ID Test Result Name Result LOINC Value
601949 Resp Syncytial Virus(RSV)ISH,T Only Bill only; no result


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Testing Algorithm

For the initial technical component only in situ hybridization (ISH) stain performed, the appropriate bill only test ID will be reflexed and charged (ISTOI). For each additional technical component only ISH stain performed, an additional bill only test ID will be reflexed and charged (ISTOA).

Mayo Clinic Laboratories | IHC Catalog Additional Information: