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Test ID SATB2 SATB2 Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Useful For

Identification of colorectal carcinoma and rectal neuroendocrine tumors

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

SATB2 IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

Special AT-rich sequence binding protein 2 (SATB2) is a transcription factor that regulates gene expression through chromatin remodeling. SATB2, when used in combination with the marker Keratin 20, may identify more than 95% of colorectal carcinoma. It may also be used to differentiate rectal neuroendocrine tumors from other neuroendocrine tumors of the gastrointestinal tract.

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; contact 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Berg KB, Schaeffer DF: SATB2 as an Immunohistochemical Marker for Colorectal Adenocarcinoma, A concise Review of Benefits and Pitfalls. Arch Pathol Lab Med 2017;141(10):1428-1433

2. Davis JL, Horvai AE: Special AT-rich sequence-binding protein 2 (SATB2) expression is sensitive but may not be specific for osteosarcoma as compared with other high-grade primary bone sarcomas. Histopathology 2016;69(1):84-90

3. Dragomir A, de Wit M, Johansson C, et al: The role of SATB2 as a diagnostic marker for tumors of colorectal origin. Am J Clin Pathol 2014;141(5):630-638

4. Magnusson K, de Wit M, Brennan DJ, et al: SATB2 in combination with cytokeratin 20 identifies over 95% of all colorectal carcinoma. Am J Surg Pathol 2011;35(7):937-948

Day(s) Performed

Monday through Friday 

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
SATB2 SATB2 IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
607601 SATB2 IHC, Tech Only Bill only; no result

Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc