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Test ID TCRF1 T-Cell Receptor Beta (TCR Beta F1) Immunostain, Technical Component Only

Ordering Guidance

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

Shipping Instructions

Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.

Specimen Required

Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Container/Tube: Immunostain Technical Only Envelope (T693)

Preferred: 2 unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block

Useful For

Classification of lymphomas

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name


Reporting Name

TCR Beta F1 IHC, Tech Only

Specimen Type


Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)

Clinical Information

T-cell receptor beta (TCR Beta F1) antibody is directed against the beta chain of the alpha/beta T-cell receptor, thus staining the majority of T lymphocytes. Staining is localized at cell membranes with weak cytoplasmic staining in some cells. Positive staining in malignant lymphomas can confirm T-cell lineage and further subtype as alpha/beta T cells.


This test includes only technical performance of the stain (no pathologist interpretation is performed). Mayo Clinic cannot provide an interpretation of tech only stains outside the context of a pathology consultation. If an interpretation is needed, refer to PATHC / Pathology Consultation for a full diagnostic evaluation or second opinion of the case. All material associated with the case is required. Additional specific stains may be requested as part of the pathology consultation, and will be performed as necessary at the discretion of the Mayo pathologist.


The positive and negative controls are verified as showing appropriate immunoreactivity and documentation is retained at Mayo Clinic Rochester. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request. Contact 855-516-8404.


Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Chan WC, Borowitz MJ, Hammami A, et al: T-cell receptor antibodies in the immunohistochemical studies of normal and malignant lymphoid cells. Cancer 1988;62(10):2118-2124

2. Chuang S, Young-Hyeh K: Cutaneous nonmycotic T- and natural killer/T-cell lymphomas: diagnostic challenges and dilemmas. J Am Acad Dermatol 2014;70:724-735

3. Krajewski AS, Myskow MW, Salter DM, et al: Diagnosis of T-cell lymphoma using beta F1, anti-T-cell receptor beta chain antibody. Histopathology 1989:15(3):239-247

4. Rudiger T, Weisenburger DD, Anderson JR, et al: Peripheral T-cell lymphoma (excluding anaplastic large-cell lymphoma): results from the Non-Hodgkin's Lymphoma Classification Project. Annals of Oncol 2002;13:140-149

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TCRF1 TCR Beta F1 IHC, Tech Only Order only;no result


Result ID Test Result Name Result LOINC Value
70872 TCR Beta F1 IHC, Tech Only Bill only; no result


If not ordering electronically, complete, print, and send an Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Mayo Clinic Laboratories | IHC Catalog Additional Information: