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Test ID TPIT T-PIT Immunostain, Technical Component Only


Ordering Guidance


This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.



Shipping Instructions


Attach the green pathology address label and the pink Immunostain Technical Only label included in the kit to the outside of the transport container.



Specimen Required


Supplies: Immunostain Technical Only Envelope (T693)

Specimen Type: Tissue

Preferred: 2 Unstained positively charged glass slide (25- x 75- x 1-mm) per test ordered; sections 4-microns thick.

Acceptable: Formalin-fixed, paraffin-embedded (FFPE) tissue block


Forms

If not ordering electronically, complete, print, and send a Immunohistochemical (IHC)/In Situ Hybridization (ISH) Stains Request (T763) with the specimen.

Useful For

Diagnosis of corticotroph, silent corticotroph, and null cell adenomas of the pituitary

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
IHTOI IHC Initial, Tech Only No No
IHTOA IHC Additional, Tech Only No No

Method Name

Immunohistochemistry (IHC)

Reporting Name

T-PIT IHC, Tech Only

Specimen Type

TECHONLY

Specimen Stability Information

Specimen Type Temperature Time Special Container
TECHONLY Ambient (preferred)
  Refrigerated 

Clinical Information

T-PIT is the transcription factor of functioning and silent corticotroph adenomas and is useful for the diagnosis of corticotroph adenoma and null cell adenoma. T-PIT may be included as part of a panel along with transcription factors SF-1 and Pit-1 to identify null cell adenomas (all negative).

Interpretation

This test includes only technical performance of the stain (no pathologist interpretation is performed). If diagnostic consultation by a pathologist is required order PATHC / Pathology Consultation.

 

The positive and negative controls are verified as showing appropriate immunoreactivity. If a control tissue is not included on the slide, a scanned image of the relevant quality control tissue is available upon request; call 855-516-8404.

 

Interpretation of this test should be performed in the context of the patient's clinical history and other diagnostic tests by a qualified pathologist.

Clinical Reference

1. Jastania R, Alsaad K, Al-Shraim M, et al: Double adenomas of the pituitary: transcription factors Pit-1, T-pit, and SF-1 identify cytogenesis and differentiation. Endocr Pathol 2005;16(3):187-194

2. Casar-Borota O, Bollerslev J, Ponten F: Immunohistochemistry for transcription factor T-Pit as a tool in diagnostics of corticotroph pituitary tumours. Pituitary 2018;21:443

3. Sjostedt E, Bollerslev J, Mulder J, et al:  A specific antibody to detect transcription factor T-Pit: a reliable marker of corticotroph cell differentiation and a tool to improve the classification of pituitary neuroendocrine tumours.  Acta Neuropathol 2017;134:675-677

4. Drummond J, Roncaroli F, Grossman A, Korbonits M: Clinical and pathological aspects of silent pituitary adenomas. J Clin Endocrinol Metab 2019;104(7):2473-2489

Day(s) Performed

Monday through Friday

Report Available

1 to 3 days

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

88342-TC, primary

88341-TC, if additional IHC

LOINC Code Information

Test ID Test Order Name Order LOINC Value
TPIT T-PIT IHC, Tech Only Order only;no result

 

Result ID Test Result Name Result LOINC Value
607890 T-PIT IHC, Tech Only Bill only; no result
Mayo Clinic Laboratories | IHC Catalog Additional Information:

mcl-ihc